Year: 2014 | Month: June | Volume 4 | Issue 1
Antifungal Activity of Bacteriocins of Lactobacillus plantarum MTCC 9503 Purified using Diatomite Calcium Silicate
Abstract:
Bacteriocins as antimicrobial metabolites from lactic acid bacteria has potential to find application as a biopreservative agent both in processed as well as fresh food. The present investigation was performed to elucidate the antifungal activity of bacteriocins of Lactobacillus plantarum MTCC 9503 against two common food spoilage fungi, Aspergillus awamori and Penicillium citrinum. Bacteriocins were purified to contain 1,00,000 AU/g of food grade adsorbing agent Diatomite calcium silicate (Micro-Cel). Antifungal efficacy was determined by observing fungal growth, spore viability and spore germination in presence of 2000 AU/ml of bacteriocin preparation. A decrease of 46% (A awamori) and 34% (P citrinum) of fungal mat, on dry weight basis was observed over the control after 10 days of incubation. Spore viability of P citrinum decreased by 20.3% and that of A awamori by 24.2% after 30h of incubation. Control runs recorded 44% spore germination compared to 29.7% (P citrinum) and 27.6% (A awamori) in trial runs after 18h of incubation. Analysis using Sigma-Statistics (One way ANOVA) indicated statistical significance of differences in control and trial results at p< 0.05.
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