Year: 2019 | Month: June | Volume 9 | Issue 1

Prevalence of Pesticide Residues in Pond Water Samples at some Districts of Haryana


DOI:June

Abstract:

The study is assessment of pesticide residues in water bodies/ponds of agriculture intensive areas, as the principal source of pesticides in crops and animal /poultry feed are soil and water. The farmers dump the remaining pesticide solutions and wash the containers with pond water near to their fields after spraying the pesticide on their crops. These measurements can be used as baseline levels to monitor and to predict their impact on the population of the area. The determination of organochlorine (OC), pyrethroids and organophosphorous (OP) insecticide residues was carried out in the samples collected from different village ponds of six districts of Haryana and analyzed at Department of Veterinary Public Health and Epidemiology, COVS, LUVAS, Hisar. A gas chromatograph equipped with an electron capture detector (GC-ECD) for OC and pyrethroids and nitrogen phosphorous detector (GC–NPD) for OP’s was employed for analysis. The testing protocol was standardized w.r.t. system precision, specificity and accuracy. The system precision values indicated a good consistency in response by the GC instrument used during present study. A good linearity was noted for standards and spiked tissue samples. Absence of interfering peaks in blank samples indicates good specificity of extraction and clean up method. Accuracy and precision of the method were in accepted range in comparison with international guidelines. Out of 50 samples, none of the sample was found to be positive for OC and Pyrethroid pesticide residues; but 16 pond water samples were detected with OP pesticide residues with prevalence rate of 32%. Monocrotophos and chlorpyriphos were detected in a maximum of eight samples each. Four samples out of 5 from Rohtak, 3 out of 5 from Jind, 3 out of 12 from Mahendergarh and 2 out of 8 from Hisar were found positive for different OP pesticide residues. All the 10 pond water samples from Ambala were found to be below detectable limit for all of the OP pesticides in study. The concentration of 16 positive samples was compared with the MRL’s of drinking water, and it was observed that 4 samples, 2 each from Rohtak and Jind for monocrotophos (3.99 & 1.99 ηg/ml from Rohtak and 1.82 & 6.88 ηg/ml from Jind) violated the normal MRL values (0.5 ηg/ml). The investigation revealed that the pond water samples of Rohtak and Sirsa were found to be most contaminated with OP pesticides, mainly with Monocrotophos followed by Chlorpyriphos which indicates the excessive and irresponsible use of these pesticides in these two districts.



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